Virus:Article Title: Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cad99C Lee et al., 2015 RRID: AB_2568534 Mouse monoclonal anti-V5 Invitrogen Cat#R960-25; RRID: AB_2556564 Rabbit polyclonal anti-FLAG Sigma-Aldrich Cat#F7425; RRID: AB_439687 Mouse monoclonal anti-Rab7 Abcam Cat#ab50533; RRID: AB_882241 Rabbit polyclonal anti-LAMP1 Abcam Cat#ab30687; RRID: AB_775973 Rabbit monoclonal anti-phospho-AMPKa Cell Signaling Technology Cat#2535; RRID: AB_331250 Rabbit anti-LacZ MP Biomedicals Cat#559761; RRID: AB_2687418 Anti-b-actin Cell Signaling Technology Cat#3700; RRID: AB_2242334 Alexa 568 goat anti-mouse IgG Invitrogen Cat#A11004; RRID: AB_141371 Alexa 568 goat anti-rabbit IgG Invitrogen Cat#A11011; RRID: AB_143157 Alexa 488 goat anti-rabbit IgG Invitrogen Cat#A11008; RRID: AB_143165 Bacterial and Virus Strains Erwinia carotovora subsp. carotovora 15 (Ecc15) Bruno Lemaitre Lab. École polytechnique fédérale de Lausanne N/A Chemicals, Peptides, and Recombinant Proteins HOCl-specific rhodamine-based R19S dye Lee et al., 2013 N/A Uridine Sigma-Aldrich Cat#U3750 Mifepristone Sigma-Aldrich Cat#M8046 MG-132 Sigma-Aldrich Cat#M7449 3-methyladenine (3-MA) Sigma-Aldrich Cat#M9281 AICAR Sigma-Aldrich Cat#A9978 Rapamycin Sigma-Aldrich Cat#R0395 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 40,6-Diamidino-2-phenylindole dihydrochloride(DAPI) Sigma-Aldirich Cat#D9542 Critical Commercial Assays Illumina Truseq RNA Sample Prep Kit Illumina RS-930-1012 Illumina True-seq RNA sample prep kit v2 Illumina RS-122-9001 SensiFAST SYBR Hi-ROX Kit Bioline Cat#BIO-92020 FuGENE6 Transfection Reagent Promega CatE2691 MATCHMAKER system 2 kit Clontech N/A Deposited Data Raw and analyzed data This paper GEO: GSE99642 D. melanogaster reference genome (NCBI build 5.3) NCBI Genome https://www.ncbi.nlm.nih.gov/ genome/ Experimental Models: Cell Lines D. melanogaster: Cell line S2 ThermoFisher Cat#R69007 Experimental Models: Organisms/Strains nos-Cas9 NIG-FLY CAS-0001 Ex-lacZ Bloomington Drosophila stock center (BDSC) #44248; RRID: BDSC_44248 Lsd2-YFP Kyoto Stock Center Drosophila Genomics and Genetic Resources (DGGR) #115181; RRID: DGGR_115181 (Continued on next page) Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NOPO / Laura A. Lee Lab, University of Vanderbilt University School of Medicine N/A TRAF3 / This paper N/A Transgene carrying TRAF3 genomic fragment This paper N/A UAS-ACC BDSC #63224; RRID: BDSC_63224 UAS-ACC-RNAi Vienna Drosophila Resource Center (VDRC) #v108631 UAS-AKT-CA Jun Hee Lee Lab, University of Michigan N/A UAS-AKT-RNAi VDRC #v103703 UAS-AMPK BDSC #32108; RRID: BDSC_32108 UAS-AMPK BDSC #32182; RRID: BDSC_32182 UAS-AMPK-RNAi VDRC #v1827 UAS-ATG1-RNAi VDRC #v16133 UAS-ATG8-RFP Thomas P. Neufeld Lab, University of Minnesota N/A UAS-BEN-RNAi VDRC #v109638 UAS-BMM Jiwon Shim Lab, Hanyang University N/A UAS-BMM-RNAi VDRC #v37880 UAS-Cad99C-GFP This laboratory N/A UAS-DUOX-RNAi This laboratory N/A UAS-FAS-RNAi VDRC #v108339 UAS-G6PD William C. Orr Lab, Southern Methodist University UAS-G6PD 4C UAS-MEKK1-RNAi VDRC #v25528 UAS-NOPO-RNAi This paper N/A UAS-NOPO-WT-FLAG_pMTa This paper N/A UAS-RHEB Jun Hee Lee Lab, University of Michigan N/A UAS-RHEB-RNAi SHared Information of GENetic Resources (SHIGEN) #1081R-2 UAS-S6K-CA Scott Pletcher Lab, University of Michigan N/A UAS-S6K-DN Scott Pletcher Lab, University of Michigan N/A UAS-SREBP Jongkyeong Chung Lab, Seoul National University N/A UAS-SREBP-RNAi Jongkyeong Chung Lab, Seoul National University N/A UAS-TOR-DN Scott Pletcher Lab, University of Michigan N/A UAS-TRAF1-RNAi VDRC #v21214 UAS-TRAF2-RNAi VDRC #v16125 UAS-TRAF3-RNAi This paper N/A UAS-TRAF3-V5_pMTa This paper N/A UAS-TSC2 Scott Pletcher Lab, University of Michigan N/A UAS-TSC2-RNAi VDRC #v6313 UAS-UEV1A-RNAi VDRC #v107465 UAS-WTS Ryan S. Udan Lab, Baylor College of Medicine N/A UAS-WTS-RNAi VDRC #v106174 UAS-Yorkie-RNAi VDRC #v104523 NP1-GAL Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A NP1-GAL4;Tub-GAL80ts Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A (Continued on next page) e2 Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018
Recombinant:Article Title: Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cad99C Lee et al., 2015 RRID: AB_2568534 Mouse monoclonal anti-V5 Invitrogen Cat#R960-25; RRID: AB_2556564 Rabbit polyclonal anti-FLAG Sigma-Aldrich Cat#F7425; RRID: AB_439687 Mouse monoclonal anti-Rab7 Abcam Cat#ab50533; RRID: AB_882241 Rabbit polyclonal anti-LAMP1 Abcam Cat#ab30687; RRID: AB_775973 Rabbit monoclonal anti-phospho-AMPKa Cell Signaling Technology Cat#2535; RRID: AB_331250 Rabbit anti-LacZ MP Biomedicals Cat#559761; RRID: AB_2687418 Anti-b-actin Cell Signaling Technology Cat#3700; RRID: AB_2242334 Alexa 568 goat anti-mouse IgG Invitrogen Cat#A11004; RRID: AB_141371 Alexa 568 goat anti-rabbit IgG Invitrogen Cat#A11011; RRID: AB_143157 Alexa 488 goat anti-rabbit IgG Invitrogen Cat#A11008; RRID: AB_143165 Bacterial and Virus Strains Erwinia carotovora subsp. carotovora 15 (Ecc15) Bruno Lemaitre Lab. École polytechnique fédérale de Lausanne N/A Chemicals, Peptides, and Recombinant Proteins HOCl-specific rhodamine-based R19S dye Lee et al., 2013 N/A Uridine Sigma-Aldrich Cat#U3750 Mifepristone Sigma-Aldrich Cat#M8046 MG-132 Sigma-Aldrich Cat#M7449 3-methyladenine (3-MA) Sigma-Aldrich Cat#M9281 AICAR Sigma-Aldrich Cat#A9978 Rapamycin Sigma-Aldrich Cat#R0395 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 40,6-Diamidino-2-phenylindole dihydrochloride(DAPI) Sigma-Aldirich Cat#D9542 Critical Commercial Assays Illumina Truseq RNA Sample Prep Kit Illumina RS-930-1012 Illumina True-seq RNA sample prep kit v2 Illumina RS-122-9001 SensiFAST SYBR Hi-ROX Kit Bioline Cat#BIO-92020 FuGENE6 Transfection Reagent Promega CatE2691 MATCHMAKER system 2 kit Clontech N/A Deposited Data Raw and analyzed data This paper GEO: GSE99642 D. melanogaster reference genome (NCBI build 5.3) NCBI Genome https://www.ncbi.nlm.nih.gov/ genome/ Experimental Models: Cell Lines D. melanogaster: Cell line S2 ThermoFisher Cat#R69007 Experimental Models: Organisms/Strains nos-Cas9 NIG-FLY CAS-0001 Ex-lacZ Bloomington Drosophila stock center (BDSC) #44248; RRID: BDSC_44248 Lsd2-YFP Kyoto Stock Center Drosophila Genomics and Genetic Resources (DGGR) #115181; RRID: DGGR_115181 (Continued on next page) Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NOPO / Laura A. Lee Lab, University of Vanderbilt University School of Medicine N/A TRAF3 / This paper N/A Transgene carrying TRAF3 genomic fragment This paper N/A UAS-ACC BDSC #63224; RRID: BDSC_63224 UAS-ACC-RNAi Vienna Drosophila Resource Center (VDRC) #v108631 UAS-AKT-CA Jun Hee Lee Lab, University of Michigan N/A UAS-AKT-RNAi VDRC #v103703 UAS-AMPK BDSC #32108; RRID: BDSC_32108 UAS-AMPK BDSC #32182; RRID: BDSC_32182 UAS-AMPK-RNAi VDRC #v1827 UAS-ATG1-RNAi VDRC #v16133 UAS-ATG8-RFP Thomas P. Neufeld Lab, University of Minnesota N/A UAS-BEN-RNAi VDRC #v109638 UAS-BMM Jiwon Shim Lab, Hanyang University N/A UAS-BMM-RNAi VDRC #v37880 UAS-Cad99C-GFP This laboratory N/A UAS-DUOX-RNAi This laboratory N/A UAS-FAS-RNAi VDRC #v108339 UAS-G6PD William C. Orr Lab, Southern Methodist University UAS-G6PD 4C UAS-MEKK1-RNAi VDRC #v25528 UAS-NOPO-RNAi This paper N/A UAS-NOPO-WT-FLAG_pMTa This paper N/A UAS-RHEB Jun Hee Lee Lab, University of Michigan N/A UAS-RHEB-RNAi SHared Information of GENetic Resources (SHIGEN) #1081R-2 UAS-S6K-CA Scott Pletcher Lab, University of Michigan N/A UAS-S6K-DN Scott Pletcher Lab, University of Michigan N/A UAS-SREBP Jongkyeong Chung Lab, Seoul National University N/A UAS-SREBP-RNAi Jongkyeong Chung Lab, Seoul National University N/A UAS-TOR-DN Scott Pletcher Lab, University of Michigan N/A UAS-TRAF1-RNAi VDRC #v21214 UAS-TRAF2-RNAi VDRC #v16125 UAS-TRAF3-RNAi This paper N/A UAS-TRAF3-V5_pMTa This paper N/A UAS-TSC2 Scott Pletcher Lab, University of Michigan N/A UAS-TSC2-RNAi VDRC #v6313 UAS-UEV1A-RNAi VDRC #v107465 UAS-WTS Ryan S. Udan Lab, Baylor College of Medicine N/A UAS-WTS-RNAi VDRC #v106174 UAS-Yorkie-RNAi VDRC #v104523 NP1-GAL Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A NP1-GAL4;Tub-GAL80ts Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A (Continued on next page) e2 Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018
Sample Prep:Article Title: Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cad99C Lee et al., 2015 RRID: AB_2568534 Mouse monoclonal anti-V5 Invitrogen Cat#R960-25; RRID: AB_2556564 Rabbit polyclonal anti-FLAG Sigma-Aldrich Cat#F7425; RRID: AB_439687 Mouse monoclonal anti-Rab7 Abcam Cat#ab50533; RRID: AB_882241 Rabbit polyclonal anti-LAMP1 Abcam Cat#ab30687; RRID: AB_775973 Rabbit monoclonal anti-phospho-AMPKa Cell Signaling Technology Cat#2535; RRID: AB_331250 Rabbit anti-LacZ MP Biomedicals Cat#559761; RRID: AB_2687418 Anti-b-actin Cell Signaling Technology Cat#3700; RRID: AB_2242334 Alexa 568 goat anti-mouse IgG Invitrogen Cat#A11004; RRID: AB_141371 Alexa 568 goat anti-rabbit IgG Invitrogen Cat#A11011; RRID: AB_143157 Alexa 488 goat anti-rabbit IgG Invitrogen Cat#A11008; RRID: AB_143165 Bacterial and Virus Strains Erwinia carotovora subsp. carotovora 15 (Ecc15) Bruno Lemaitre Lab. École polytechnique fédérale de Lausanne N/A Chemicals, Peptides, and Recombinant Proteins HOCl-specific rhodamine-based R19S dye Lee et al., 2013 N/A Uridine Sigma-Aldrich Cat#U3750 Mifepristone Sigma-Aldrich Cat#M8046 MG-132 Sigma-Aldrich Cat#M7449 3-methyladenine (3-MA) Sigma-Aldrich Cat#M9281 AICAR Sigma-Aldrich Cat#A9978 Rapamycin Sigma-Aldrich Cat#R0395 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 40,6-Diamidino-2-phenylindole dihydrochloride(DAPI) Sigma-Aldirich Cat#D9542 Critical Commercial Assays Illumina Truseq RNA Sample Prep Kit Illumina RS-930-1012 Illumina True-seq RNA sample prep kit v2 Illumina RS-122-9001 SensiFAST SYBR Hi-ROX Kit Bioline Cat#BIO-92020 FuGENE6 Transfection Reagent Promega CatE2691 MATCHMAKER system 2 kit Clontech N/A Deposited Data Raw and analyzed data This paper GEO: GSE99642 D. melanogaster reference genome (NCBI build 5.3) NCBI Genome https://www.ncbi.nlm.nih.gov/ genome/ Experimental Models: Cell Lines D. melanogaster: Cell line S2 ThermoFisher Cat#R69007 Experimental Models: Organisms/Strains nos-Cas9 NIG-FLY CAS-0001 Ex-lacZ Bloomington Drosophila stock center (BDSC) #44248; RRID: BDSC_44248 Lsd2-YFP Kyoto Stock Center Drosophila Genomics and Genetic Resources (DGGR) #115181; RRID: DGGR_115181 (Continued on next page) Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NOPO / Laura A. Lee Lab, University of Vanderbilt University School of Medicine N/A TRAF3 / This paper N/A Transgene carrying TRAF3 genomic fragment This paper N/A UAS-ACC BDSC #63224; RRID: BDSC_63224 UAS-ACC-RNAi Vienna Drosophila Resource Center (VDRC) #v108631 UAS-AKT-CA Jun Hee Lee Lab, University of Michigan N/A UAS-AKT-RNAi VDRC #v103703 UAS-AMPK BDSC #32108; RRID: BDSC_32108 UAS-AMPK BDSC #32182; RRID: BDSC_32182 UAS-AMPK-RNAi VDRC #v1827 UAS-ATG1-RNAi VDRC #v16133 UAS-ATG8-RFP Thomas P. Neufeld Lab, University of Minnesota N/A UAS-BEN-RNAi VDRC #v109638 UAS-BMM Jiwon Shim Lab, Hanyang University N/A UAS-BMM-RNAi VDRC #v37880 UAS-Cad99C-GFP This laboratory N/A UAS-DUOX-RNAi This laboratory N/A UAS-FAS-RNAi VDRC #v108339 UAS-G6PD William C. Orr Lab, Southern Methodist University UAS-G6PD 4C UAS-MEKK1-RNAi VDRC #v25528 UAS-NOPO-RNAi This paper N/A UAS-NOPO-WT-FLAG_pMTa This paper N/A UAS-RHEB Jun Hee Lee Lab, University of Michigan N/A UAS-RHEB-RNAi SHared Information of GENetic Resources (SHIGEN) #1081R-2 UAS-S6K-CA Scott Pletcher Lab, University of Michigan N/A UAS-S6K-DN Scott Pletcher Lab, University of Michigan N/A UAS-SREBP Jongkyeong Chung Lab, Seoul National University N/A UAS-SREBP-RNAi Jongkyeong Chung Lab, Seoul National University N/A UAS-TOR-DN Scott Pletcher Lab, University of Michigan N/A UAS-TRAF1-RNAi VDRC #v21214 UAS-TRAF2-RNAi VDRC #v16125 UAS-TRAF3-RNAi This paper N/A UAS-TRAF3-V5_pMTa This paper N/A UAS-TSC2 Scott Pletcher Lab, University of Michigan N/A UAS-TSC2-RNAi VDRC #v6313 UAS-UEV1A-RNAi VDRC #v107465 UAS-WTS Ryan S. Udan Lab, Baylor College of Medicine N/A UAS-WTS-RNAi VDRC #v106174 UAS-Yorkie-RNAi VDRC #v104523 NP1-GAL Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A NP1-GAL4;Tub-GAL80ts Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A (Continued on next page) e2 Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018
Transfection:Article Title: Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cad99C Lee et al., 2015 RRID: AB_2568534 Mouse monoclonal anti-V5 Invitrogen Cat#R960-25; RRID: AB_2556564 Rabbit polyclonal anti-FLAG Sigma-Aldrich Cat#F7425; RRID: AB_439687 Mouse monoclonal anti-Rab7 Abcam Cat#ab50533; RRID: AB_882241 Rabbit polyclonal anti-LAMP1 Abcam Cat#ab30687; RRID: AB_775973 Rabbit monoclonal anti-phospho-AMPKa Cell Signaling Technology Cat#2535; RRID: AB_331250 Rabbit anti-LacZ MP Biomedicals Cat#559761; RRID: AB_2687418 Anti-b-actin Cell Signaling Technology Cat#3700; RRID: AB_2242334 Alexa 568 goat anti-mouse IgG Invitrogen Cat#A11004; RRID: AB_141371 Alexa 568 goat anti-rabbit IgG Invitrogen Cat#A11011; RRID: AB_143157 Alexa 488 goat anti-rabbit IgG Invitrogen Cat#A11008; RRID: AB_143165 Bacterial and Virus Strains Erwinia carotovora subsp. carotovora 15 (Ecc15) Bruno Lemaitre Lab. École polytechnique fédérale de Lausanne N/A Chemicals, Peptides, and Recombinant Proteins HOCl-specific rhodamine-based R19S dye Lee et al., 2013 N/A Uridine Sigma-Aldrich Cat#U3750 Mifepristone Sigma-Aldrich Cat#M8046 MG-132 Sigma-Aldrich Cat#M7449 3-methyladenine (3-MA) Sigma-Aldrich Cat#M9281 AICAR Sigma-Aldrich Cat#A9978 Rapamycin Sigma-Aldrich Cat#R0395 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 40,6-Diamidino-2-phenylindole dihydrochloride(DAPI) Sigma-Aldirich Cat#D9542 Critical Commercial Assays Illumina Truseq RNA Sample Prep Kit Illumina RS-930-1012 Illumina True-seq RNA sample prep kit v2 Illumina RS-122-9001 SensiFAST SYBR Hi-ROX Kit Bioline Cat#BIO-92020 FuGENE6 Transfection Reagent Promega CatE2691 MATCHMAKER system 2 kit Clontech N/A Deposited Data Raw and analyzed data This paper GEO: GSE99642 D. melanogaster reference genome (NCBI build 5.3) NCBI Genome https://www.ncbi.nlm.nih.gov/ genome/ Experimental Models: Cell Lines D. melanogaster: Cell line S2 ThermoFisher Cat#R69007 Experimental Models: Organisms/Strains nos-Cas9 NIG-FLY CAS-0001 Ex-lacZ Bloomington Drosophila stock center (BDSC) #44248; RRID: BDSC_44248 Lsd2-YFP Kyoto Stock Center Drosophila Genomics and Genetic Resources (DGGR) #115181; RRID: DGGR_115181 (Continued on next page) Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NOPO / Laura A. Lee Lab, University of Vanderbilt University School of Medicine N/A TRAF3 / This paper N/A Transgene carrying TRAF3 genomic fragment This paper N/A UAS-ACC BDSC #63224; RRID: BDSC_63224 UAS-ACC-RNAi Vienna Drosophila Resource Center (VDRC) #v108631 UAS-AKT-CA Jun Hee Lee Lab, University of Michigan N/A UAS-AKT-RNAi VDRC #v103703 UAS-AMPK BDSC #32108; RRID: BDSC_32108 UAS-AMPK BDSC #32182; RRID: BDSC_32182 UAS-AMPK-RNAi VDRC #v1827 UAS-ATG1-RNAi VDRC #v16133 UAS-ATG8-RFP Thomas P. Neufeld Lab, University of Minnesota N/A UAS-BEN-RNAi VDRC #v109638 UAS-BMM Jiwon Shim Lab, Hanyang University N/A UAS-BMM-RNAi VDRC #v37880 UAS-Cad99C-GFP This laboratory N/A UAS-DUOX-RNAi This laboratory N/A UAS-FAS-RNAi VDRC #v108339 UAS-G6PD William C. Orr Lab, Southern Methodist University UAS-G6PD 4C UAS-MEKK1-RNAi VDRC #v25528 UAS-NOPO-RNAi This paper N/A UAS-NOPO-WT-FLAG_pMTa This paper N/A UAS-RHEB Jun Hee Lee Lab, University of Michigan N/A UAS-RHEB-RNAi SHared Information of GENetic Resources (SHIGEN) #1081R-2 UAS-S6K-CA Scott Pletcher Lab, University of Michigan N/A UAS-S6K-DN Scott Pletcher Lab, University of Michigan N/A UAS-SREBP Jongkyeong Chung Lab, Seoul National University N/A UAS-SREBP-RNAi Jongkyeong Chung Lab, Seoul National University N/A UAS-TOR-DN Scott Pletcher Lab, University of Michigan N/A UAS-TRAF1-RNAi VDRC #v21214 UAS-TRAF2-RNAi VDRC #v16125 UAS-TRAF3-RNAi This paper N/A UAS-TRAF3-V5_pMTa This paper N/A UAS-TSC2 Scott Pletcher Lab, University of Michigan N/A UAS-TSC2-RNAi VDRC #v6313 UAS-UEV1A-RNAi VDRC #v107465 UAS-WTS Ryan S. Udan Lab, Baylor College of Medicine N/A UAS-WTS-RNAi VDRC #v106174 UAS-Yorkie-RNAi VDRC #v104523 NP1-GAL Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A NP1-GAL4;Tub-GAL80ts Bruce A. Edgar Lab, Center for Molecular Biology Heidelberg University (ZMBH) Alliance N/A (Continued on next page) e2 Cell Host & Microbe 23, 338–352.e1–e5, March 14, 2018
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